Molecular Formula | C20H21CaN7O7
|
Molar Mass | 511.5 |
Melting Point | 240-250°C |
Specific Rotation(α) | D21 +14.9° (c = 1 in water) |
Water Solubility | It is soluble in water, but almost insoluble in ethanol or ether; it is soluble in 0.1mol/l sodium hydroxide solution |
Appearance | powder |
Color | Yellow-white to yellow |
Storage Condition | Keep in dark place,Inert atmosphere,2-8°C |
In vitro study | Leucovorin increases the reduced levels of folate in tissues, thereby promoting the inhibition of thymidylate synthase in both mouse colon tumors. Leucovorin,Gemcitabine, Oxaliplatin and 5-fluorouracil are a powerful anti-tumor and immunomodulatory therapy that can make tumor cells suitable means to induce antigen-specific CTL responses. In 11 human colon cancer cell lines, Leucovorin(20 mM) increased cytotoxicity. In CCRF-CEM cells, Leucovorin potentiated trimethyl/Fluorouracil toxicity but not methotrexate/Fluorouracil. In tumor and normal tissues, Leucovorin(LV) is a reduced folate cofactor that competes with RTX for transport and polyglutamation, and thus can serve as a potential rescue agent. Leucovorin reverses growth inhibition and potentiates the cytotoxic effects of RTX. |
In vivo study | In mice, the administration of Leucovorin(200 mg/kg) after severe weight loss and diarrhea (twice daily for 5-7 days) prevented further weight loss and caused early recovery. Compared with the control group, Leucovorin(100 or 200 mg/kg; Once every two days, 4-6 days) reduced the concentration of RTX in the liver by 2-4 times. The combination of Leucovorin/5-FU and gastricmmune (200 mg/mL) increased the growth of DHDK12 tumors in vivo. |